DPLSM

Fast and high accuracy determination of double refraction in laser scanning microscopes

Fast and high accuracy determination of double refraction in laser scanning microscopes
Dual-fracture analysis is widely used, mainly in polarization spectroscopy, in crystalline, liquid crystal and other intelligent materials. it is commonly used in material structure research and quality control studies, but is widely used to reveal the anisotropic molecular structure of various biological samples and in many areas of medical diagnostics. dplsm_prod_462 The double-fracture and / or fracture many procedures have been used to measure the resulting phase shift on macroscopic samples. The presence of double fractures on microscopic specimens is possible in “conventional” polarization microscopes. There are also several methods known for more modern laser scanning microscopes (LSMs). Commercially available devices essentially use the imaging method used in polarization microscopes, crossed polar filters. However, in this way, only qualitative data on double-break are available, and this procedure does not take advantage of LSMk’s most important advantage, digital data recording per pixel. It provides a significantly more accurate and faster procedure using photoelastic modulators and demodulation equipment. There are currently two such procedures. equipment is known. Although these can be used to accurately determine the local values ​​of the double fracture, the measurement procedure is not widespread. One reason for this is probably the relatively high cost of the auxiliary equipment and the relatively complexity of the process. These disadvantages are eliminated by our proposed method and apparatus: a new method of microscopic determination of double refraction, by inserting a beam opener, replacing a transmitted light measurement detector with a multi-channel (quadrant) detector, which is polarized by various passive elements; the direction and magnitude of the birefringence can be determined per pixel after computer signal processing.

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